We use a multidisciplinary approach combining acute combining acute protein inactivation, 3D-live cell imaging and quantitative methods to probe for chromosome morphology and mitosis progression (see gallery). Our "acute approach" relies on a major technical advance that enables specific, rapid and efficient protein inactivation in a tissue- and/or time-dependent manner (see Pauli et al Dev Cell 2008, Oliveira et al NCB 2010 and Piskadlo et al eLife 2017). This method uses an exogenous protease (Tobacco Etch Virus, TEV) to cleave an engineered protein of interest that contains TEV cleavage sites. Additional tools for acute protein depletion are under development.
- Model systems