Oliveira lab
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OUR APPROACH

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We use a multidisciplinary approach combining acute combining acute protein inactivation, 3D-live cell imaging and quantitative methods to probe for chromosome morphology and mitosis progression (see gallery). Our "acute approach" relies on a major technical advance that enables specific, rapid and efficient protein inactivation in a tissue- and/or time-dependent manner (see Pauli et al Dev Cell 2008, Oliveira et al NCB 2010 and Piskadlo et al eLife 2017). This method uses an exogenous protease (Tobacco Etch Virus, TEV) to cleave an engineered protein of interest that contains TEV cleavage sites. Additional tools for acute protein depletion are under development.

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  • Model systems
We use mostly Drosophila melanogaster as a model system. Fruit flies are an excellent model to address chromosome structure, as they offer a powerful system for simple genetic manipulations combined with live cell imaging of many mitotic tissues in which condensed mitotic chromosomes can be easily visualised. Additionally, they offer the unique opportunity to study the consequences of aberrant chromosome structure in the context of a developing organism. 

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@IGC
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© 2022 Raquel Oliveira
  • Home
  • Research
  • Publications
    • In the news
  • People
    • Open Positions
  • Gallery
  • Photos
    • CHR 10th Anniversary
    • CHR Official photos
    • CHR Random
    • CHR Moments of Pride
    • CHR Secret Santa 2020
    • Lab Retreat Azores 2018
    • CHR 5th Anniversary
    • CHR 4th Anniversary
    • DrosTuga 2014
    • CHR 2nd anniversary
    • CHR 1st anniversary
  • Contact
  • Knitted chromosomes